Vaginas grown from four teenagers' own cells have been implanted into them.
The organs are working normally in the girls, who were aged between 13 and 18 at the time of surgery.
They were born with a syndrome that means the vagina and uterus are absent or underdeveloped.
The cells were grown on specially moulded frames.
Dr Anthony Atala, lead researcher of the US team, said: "This may represent a new option for vaginal reconstructive surgeries."
The pioneering treatment was carried out between 2005 and 2008.
All the patients have been able to have normal sex lives.
One in every 5,000 women is born without a vagina, cervix or womb.
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